ANALYSIS OF MTDNA SEQUENCES OF EUROPEAN GRAYLING, THYMALLUS THYMALLUS, FROM SOUTHWESTERN POLAND
Łukasz Jurczyk, Paweł Brzuzan
University of Warmia and Mazury in Olsztyn, Poland
ABSTRACT. Nucleotide sequences of the partial mitochondrial DNA (mtDNA) control region and flanking tRNAPhe gene (a total of 150bp) were determined in eight specimens of European grayling, Thymallus thymallus, representing five rivers in southwestern Poland. Two haplotypes, A and B, were identified which differed from each other by two nucleotide substitutions. Haplotype A was detected in fish from two rivers, the Zadrna and Bóbr, whereas haplotype B was detected in fish from three others, the Widna, Biala Gluchołazka and Metuja. The different phylogenetic position of the mtDNA haplotypes observed in samples from rivers which are in close proximity may suggest genetic structuring of grayling populations in the region, but further sampling is required to test this hypothesis.
Key words: D-LOOP, mtDNA VARIATION, EUROPEAN GRAYLING (THYMALLUS THYMALLUS)
Dr hab. Paweł Brzuzan, Uniwersytet Warmińsko-Mazurski, Zakład Genetyki Ewolucyjnej, ul. Oczapowskiego 5;
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