Arch. Pol. Fish.     Vol. 8     Fasc. 2            2000      p. 171-180


SOME HUMORAL EFFECTS IN SIBERIAN STURGEON (ACIPENSER BAERI BRANDT) AFTER LYSOZYME DIMER APPLICATION IN BATH

Halina Kolman1, Ryszard Kolman2, Andrzej K. Siwicki1,2, Bożena Szczepkowska2

1 University Warmia and Mazury in Olsztyn
2 The Stanisław Sakowicz Inland Fisheries Institute in Olsztyn

ABSTRACT. The present study was undertaken to evaluate humoral indices response in sturgeons reared under controlled conditions and treated with exogenous lysozyme dimer in immersion. Fish of average body weight 40±4 g were bathed for 30 minutes in 0.1 mg l-1 of dimer (Lydium KLP-602). After the treatment, blood was sampled from the fish for 7 weeks, at weekly intervals. Lysozyme activity, the level of g-globulins, ceruloplasmin, and total protein were measured in blood serum. Average body weight of fish increased 5 fold (to 210±12 g) during the experiment. In treated fish the bacteridytie activity of endogenous lisozyme, g-globulins, and ceruloplasmin levels increased already in the first week after immersion by 27%, 40%, and 60% respectively. Increase of these parameters was observed until the end of the experiment. The results indicate that lysozyme dimer stimulated humoral responses in fish.

Keywords: CHONDROSTEI, HUMORAL IMMUNITY, g-GLOBULINS, LYSOZYME, CERULOPLASMIN, LYDIUM KLP-602