KOMUNIKATY RYBACKIE

Nr 6 (119)/2010, 16-20

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COMPARITIVE CHARACTERIZATION OF SELECTED PARAMETERS OF MILT QUALITY IN FOUR BREEDING STRAINS OF CARP, CYPRINUS CARPIO L.

Beata Irena Cejko, Jan Glogowski

ABSTRACT. Values of selected parameters of milt from carp, Cyprinus carpio L., representing four breeding strains – the Lithuanian strain B (n = 12); the Hungarian strain W (n = 16), the Ukrainian strain Ur (n = 16), and the Polish strain 6 (n = 14) collected during the spawning season after stimulation with Ovopel (1 pellet/kg) were compared. Spermatozoa motility (%), their concentration in milt (billion/ml), total protein content in seminal plasma (mg/ml), as well as its osmolality (mOsm/kg) were determined. The activity of lactate dehydrogenase (LDH), acid phosphatase, and anti-proteolytic activity (APA) were also determined. Values obtained for the activity of the analyzed enzymes are presented in U/l. Significant differences were observed among the basic parameters of milt only in total protein content between males of the Ur and W strains (P < 0.01) and strain 6 (P < 0.05). Significant differences were not noted in the activity of LDH, AcP, or APA (P>0.05). High correlation coefficients (R = 0.862, P < 0.001 and R = 0.667, P < 0.01) between APA activity and total protein content in the seminal plasma of males from strain 6 suggest that protective proteins might constitute the main protein fraction in the seminal plasma of these fish.

Keywords: carp strains, milt, spermatozoa motility, acid phosphatase, lactate dehydrogenase, anti-proteolitic activity


Przyjęto po recenzji 15.10.2010 r.